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[INTENDED USE]
The Brucella Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Brucella Antibody (Brucella Ab) in cattle's and sheep's serum, plasma, or whole blood. Assay Time: 5-10 minutes Specimen: serum, plasma, or whole blood.
[KIT COMPONENTS]
|
Components |
Contents |
|
(1)Bovine Brucella Virus antigen rapid test cassette |
25bag/box(1test/foil bag) |
|
(2)Buffer |
25pcs/box |
|
(3)Dropper |
25pcs/box |
|
(4)Instruction |
1pcs/box |
[PRINCIPLE]
The Brucella Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella LPS antigens. If there are anti-Brucella antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Brucella antibodies in the specimen.
[TEST PROCEDURE]
Collect cattle's fresh whole blood, serum or plasma specimen for use. Immediately apply the testing once collected the specimen.
Take out the test device from the foil pouch and place it horizontally.
Place 2drop (approx. 20μL,by the capillary dropper) of serum or plasma specimen into an assay buffer tube. Mix well and directly use in the assay.
Place 2-3drops (approx.60-80μL,by the disposable dropper) of assay buffer into the sample hole.
Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
[Results judgment]
Positive (+): Both of T and C lines appear
Negative (-): C line appears while no T line appeared
Invalid: If the C line does not appear, indicating that the operation process is incorrect or the test cassette is invalid.
[Storage and expiry date]
This product should be stored at 2～30℃, in a cool, dark, dry place, never frozen;
See the outer packaging for the expiry date and batch number
[Note]
(1) Do not use the test kit if the packaging is damaged or has not expired.
(2) The test kit should not be frozen during storage or transportation, and avoid direct sunlight.
(3) The operator should not eat, drink or smoke during the test.
(4) When operating, operators should wear gloves and wash their hands after handling.
(5) After the test is completed, the test card and other items shall be harmlessly disposed together with the test samples.
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