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[INTENDED USE]
The Avian Salmonella spp. Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Avian Salmonella spp. antigen (Salmonella Ag) in feces from bird's cloaca or ground. Eggs contaminated with Salmonella could also be detected with the auxiliary of enrichment broth.
[KIT COMPONENTS]
|
Components |
Contents |
|
(1)Avian Salmonella spp antigen rapid test cassette |
25bag/box(1test/foil bag) |
|
(2)Swab |
25pcs/box |
|
(3)Buffer |
25pcs/box |
|
(4)Dropper |
25pcs/box |
|
(5)Instruction |
1pcs/box |
[PRINCIPLE]
The Avian Salmonella spp. Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is NDV antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Avian Salmonella spp. antigen in the specimen.
[TEST PROCEDURE]
Collect bird's feces from the cloaca or ground with the cotton swab and Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
If collecting the egg specimen, the specimen should be enriched in an enrichment broth and inactivated before running the assay. Refer to the National Standard Procedures or AOAC suggested method to enrich the Salmonella-suspicious specimen.
Take out the test device from the foil pouch and place it horizontally.
Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole "S" of the test device.
[Results judgment]
Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
Negative (-): Only clear C line appear. No T line.
Invalid: No colored line appears in C zone. No matter if T line appears.
[Storage and expiry date]
This product should be stored at 2～30℃, in a cool, dark, dry place, never frozen;
The validity period is 12 months. See the outer packaging for the expiry date and batch number
[Note]
(1) Do not use the test kit if the packaging is damaged or has not expired.
(2) The test kit should not be frozen during storage or transportation, and avoid direct sunlight.
(3) The operator should not eat, drink or smoke during the test.
(4) When operating, operators should wear gloves and wash their hands after handling.
(5) After the test is completed, the test card and other items shall be harmlessly disposed together with the test samples.
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