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[INTENDED USE]
ASFV Ag Rapid kit is a lateral flow chromatographic immunoassay for detection of African swine fever virus (ASFV) antigen in blood of porcine.
This is diagnostic kit to detect ASFV antigen using porcine whole blood.
You can drop the specimen onto the sample hole and add dilution buffer.
If there are ASFV antigens in the porcine whole blood, these antigens bind to ASFV antibody-fluorescent particle conjugates and move on the membrane by capillary forces. And then the reaction appears on the test line when the result is read using a fluorescent reader.
This test kit is the diagnostic reagent that can detect ASFV antigens quickly and simply at 15 minutes (reaction time) after infection of Samples
[KIT COMPONENTS]
|
Components |
Contents |
|
(1)African Swine Fever Virus Rapid Test Kit |
50bag/box(1test/foil bag) |
|
(2)Buffer |
1bottle/box(10ml/bottle) |
|
(3)Dropper |
50pcs/box |
|
(4)Instruction |
1pcs/box |
[SAMPLE PREPARATION]
Before the test, be sure to read yourself with TEST PROCEDURE and PRECAUTIONS in user manual
1. Blood collection
Blood should be collected from the jugular or ear vein of the suspected or infected pigs using a sterile disposable syringe.
2. Sample preparation
1) Sample's ID should be marked on the surface of the sample tube (Eppendorf tube).
2) The fresh blood should be mixed with anticoagulant (EDTA) and be dropped into the eppendorf tube with 0.5~1 ㎖.
3) Kept at 2~8°C within 48 hours.
4) The person who collect blood and the person who test should be separated and do not contact directly each other.
5) The test should be done at the separated, clean and flat place avoiding contact and contamination of the device
[TEST PROCEDURE]
Method 1: For whole blood or anticoagulant blood
(1) Take out the test cassette from the sealed bag and use it within one hour after been opened.
(2) Place the product on a flat desk.
(3) Absorb the blood sample with the dropper and add 1 drop to the sample hole..
(4) Add 2 drops of buffer to the sample hole of the test cassette, and start timing.
(5) Interpret the result within 10 to 15 minutes.
Method 2: For serum
(1) Take out the test cassette from the sealed bag and use it within one hour after been opened.
(2) Place the product on a flat desk.
(3) Take 1 mL of collected porcine whole blood sample into a 1.5 mL centrifuge tube, centrifuge at 3500r/min for 5 minutes, take the upper serum sample with the dropper, add 1 drop to the sample hole.
(4) Add 2 drops of buffer to the sample hole of the test cassette, and start timing.
(5) Interpret the result within 10 to 15 minutes.
[Results judgment]
Positive (+): Both of T and C lines appear , indicating that the sample contains African swine fever virus.
Negative (-): C line appears while no T line appeared, indicating that the sample does not contain African swine fever virus.
Invalid: If the C line does not appear, indicating that the operation process is incorrect or the test cassette is invalid.
[Storage and expiry date]
This product should be stored at 2～30℃, in a cool, dark, dry place, never frozen;
The validity period is 12 months. See the outer packaging for the expiry date and batch number
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